Canadian National (CN) and Canadian Pacific Railway (CPR) handle the majority of the Port's cargo across their extensive Canadian and U.S. networks. These transcontinental networks are enhanced by Burlington Northern Santa Fe railway (BNSF).

By road, the Port of Vancouver offers easy access to the Trans-Canada Highway and U.S. Interstate highway system with interconnecting service provided by major trucking lines. Trucks can provide third and fourth morning delivery to major centres in Eastern Canada and the United States.